10.5
La Colonna Winogradsky
Una colonna di Winogradsky fornisce uno strumento potente per studiare l'ecologia microbica e le interazioni metaboliche in un ambiente stratificato e…
Una colonna di Winogradsky è un ecosistema microbico artificiale auto-organizzato per studiare la diversità microbica e il metabolismo.
È costruito riempiendo un cilindro di vetro a metà con sedimenti ricchi di organici.
La carta triturata viene aggiunta come fonte di carbonio, il gesso come fonte di solfato e il carbonato di calcio come tampon.
La colonna è piena d'acqua ed esposta alla luce.
Col tempo, nella colonna si sviluppano strati microbici distinti, creando gradienti di ossigeno e zolfo.
In cima, i cianobatteri eseguono la fotosintesi ossigeno, producendo ossigeno e mantenendo la zona superiore aerobica.
Sotto questo, i batteri chemolitotrofici dello zolfo utilizzano questo ossigeno per ossidare il solfuro di idrogeno.
Nelle zone anossiche inferiori, batteri di zolfo viola e verde eseguono la fotosintesi anossigeno, utilizzando il solfuro di idrogeno per fissare l'anidride carbonica.
Negli strati anossici più profondi, batteri anaerobi come riduttori di solfato e fermentatori riducono il solfato in solfuro di idrogeno, che diffonde verso l'alto ed è utilizzato dai batteri ossidanti dello zolfo, creando un ciclo verticale dello zolfo.
View the full transcript and gain access to JoVE Core videos
Q1: What is a Winogradsky column and how is it constructed?
A Winogradsky column is a self-organizing artificial microbial ecosystem built in a glass cylinder filled halfway with organic-rich sediment mixed with shredded paper as a carbon source, gypsum for sulfate, and calcium carbonate as a buffer. The cylinder is filled with water, sealed, and exposed to light. Over time, distinct microbial layers develop, creating oxygen and sulfur gradients that support diverse metabolic processes.
Q2: How do oxygen gradients form in a Winogradsky column?
Cyanobacteria at the column's top perform oxygenic photosynthesis, producing oxygen and maintaining an aerobic upper zone. Below this, chemolithotrophic sulfur bacteria use the available oxygen to oxidize hydrogen sulfide. Deeper layers become progressively anoxic as oxygen is consumed, creating a vertical oxygen gradient that supports different microbial metabolisms.
Q3: What role do sulfur bacteria play in the Winogradsky column?
Sulfur bacteria occupy multiple zones based on oxygen availability. Colorless sulfur bacteria in microaerophilic zones oxidize hydrogen sulfide using limited oxygen. Purple and green sulfur bacteria in anoxic zones perform anoxygenic photosynthesis, using hydrogen sulfide as an electron donor to fix carbon dioxide, contributing to primary production without oxygen.
Q4: How does the sulfur cycle operate within a Winogradsky column?
At the column's base, anaerobic bacteria such as sulfate reducers decompose organic matter and reduce sulfate to hydrogen sulfide. This hydrogen sulfide diffuses upward and serves as an energy source for sulfur-oxidizing and photosynthetic bacteria in upper layers. This creates a closed-loop sulfur cycle sustaining the entire microbial community through vertical chemical cycling.
Q5: What types of photosynthetic bacteria exist in different Winogradsky column zones?
Oxygenic phototrophs like cyanobacteria dominate the top aerobic zone, fixing carbon dioxide with light energy. Purple nonsulfur bacteria occupy microaerophilic zones using photoheterotrophy. Purple and green sulfur bacteria thrive in strictly anoxic deeper zones, performing anoxygenic photosynthesis. Each group occupies a distinct ecological niche based on light and oxygen availability.
Q6: Why is a Winogradsky column useful for studying microbial ecology?
The Winogradsky column replicates complex biogeochemical gradients found in natural sediments within a controlled, transparent system. It allows researchers to observe microbial succession and metabolic interactions over time, demonstrating how diverse microorganisms depend on each other through nutrient cycling. This makes it an invaluable model for understanding environmental microbiology and ecosystem complexity.
Q7: What materials are added to a Winogradsky column and what purpose does each serve?
Shredded paper provides a carbon source for microbial metabolism. Gypsum supplies sulfate, which anaerobic bacteria reduce to hydrogen sulfide. Calcium carbonate acts as a pH buffer, maintaining stable conditions. Pond water introduces diverse microorganisms. Together, these components create the chemical and biological foundation for establishing stratified microbial communities.