Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding

Abstract

Source: Zhang, M. et al., The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea. J. Vis. Exp. (2018)

This video demonstrates the establishment of rice plant leaf infection through wounding inoculation with Magnaporthe grisea mycelium. The fungal spore-derived germ tube penetrates the epidermal layer, resulting in tissue infection and lesions on the leaves. This method aids pathotype screening and improves plant resistance understanding for molecular breeding.

Protocol

1. Fungal culture for M.grisea

1. Prepare the oatmeal tomato agar (OTA) culture medium for fungal strains.
2. Weigh 30 - 50 g of oatmeal, add this to 800 mL of distilled/deionized water (ddH₂O), and boil the mixture for 30 min in the electric pot.
3. Filter the boiled oatmeal juice into the beaker through a piece of gauze.
4. Add 150 mL of tomato juice and 20 g of agar to the filtrate in the beaker and add ddH₂O up to 1,000 mL.

2. Preparation of experimental materials

1. Soak about 50 seeds of rice (Oryza sativa) cult....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Agar	AOBOX Biotechnology (China)	01-023
Filter paper	GE Healthcare brand(Sweden)	10311387
Tween-20	Coolaber (China)	CT11551-100ml
Culture dish	Thermofisher(Amercia)	150326
Vacuum pump	Leybold	D25B
Dissection needle	FST	26000-35
Incubator	MEMMERT	PYX313