Erratum: Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Sommario

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Abstract

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Protocollo

È stato emesso un erratum per Ricolonizzazione da cellule epiteliali e preparazione di impalcature di matrice extracellulare da roditore per lo sviluppo del tessuto renale.

Le linee cellulari umane RCTEC/RCTE utilizzate in questo studio e in Caralt et al., 2015, Uzarski et al., 2015 sono state originariamente fornite dal dottor Loghman-Adham (allora presso la St. Louis University) al coautore dottor Wandinger-Ness (University of New Mexico) tramite un MTA nel 2001. Le cellule RCTEC/RCTE umane immortalizzate con SV40 sono state caratterizzate come appartenenti alla linea di cellule del tubulo distale, come descritto in Loghman-Adham et al., 2003. Sulla base di recenti sequenziamenti del DNA mediante ripetizioni in tandem corte (STR) (eseguiti da IDEXX BioResearch) delle passaggi più precoci delle linee cellulari RCTEC/RCTE, è emerso che le cellule erano di linea mista. Ulteriori analisi dei prodotti PCR mediante elettroforesi capillare QIAxcel hanno dimostrato la presenza di un prodotto canino. Il prodotto è stato sequenziato ed è stato stabilito che fosse di origine canina. Un insieme di marcatori STR specifici per il cane è stato confrontato con il campione, mostrando che il profilo genetico del campione presentava una identità del 92% con la linea cellulare MDCK.  Le linee cellulari successive utilizzate nella presente pubblicazione sono state profilate mediante STR e hanno mostrato una deriva verso una linea MDCK al 100%. Nonostante l'origine canina, anziché umana come precedentemente ritenuto, MDCK è anch'essa una linea di cellule epiteliali del tubulo distale. Per questo motivo, le precedenti interpretazioni e conclusioni ottenute utilizzando queste cellule in questo studio e in Caralt et al., 2015, Uzarski et al., 2015 rimangono valide, ma per garantire rigore, riproducibilità e validazione sperimentale da parte di altri, riportiamo questo errore di identificazione. Questa informazione verrà inoltre segnalata per l'inserimento nel database del Comitato Internazionale per l'Autenticazione delle Linee Cellulari (ICLAC) (http://iclac.org).