Atr-ftir Analysis
Atr-ftir Analysis - Related Videos
10:06
Characterization Of Multi-layered Fish Scales (Atractosteus spatula) Using Nanoindentation, X-ray CT, FTIR, and SEM
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Cited by 15 •
2014
This paper presents the methods used for probing spatially correlated chemical, structural, and mechanical properties of the multilayered scale of Atractosteus spatula (A. spatula) using nanoindentation, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and X-ray computed tomography (X-ray CT). The experimental results have been used to investigate the design principles of protective biological materials.
01:13
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview
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2024
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples. The ATR process begins by directing a beam...
08:27
Sampling and Identification of Microplastics in Groundwater
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2025
Here, we provide a detailed description of sampling groundwater from a borehole for microplastics analysis using a patented sampling system developed for this purpose. The protocol details the methods for sampling microplastics from boreholes, as well as the separation and chemical identification of microplastics.
08:11
Infrared Spectroscopy
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2023
Source: Vy M. Dong and Zhiwei Chen, Department of Chemistry, University of California, Irvine, CA This experiment will demonstrate the use of infrared (IR) spectroscopy (also known as vibrational spectroscopy) to elucidate the identity of an unknown compound by identifying the functional group(s) present. IR spectra will be obtained on an IR spectrometer using the attenuated total reflection (ATR) sampling technique with a neat sample of the unknown.
10:16
Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
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Cited by 186 •
2016
The protocol below describes the methodology for: microplastics sampling on the sea surface, separation of microplastic and chemical identification of particles. This protocol is in line with the recommendations for microplastics monitoring published by the MSFD Technical Subgroup on Marine Litter.
FAQs
The internal reflection element, often a crystal, couples infrared radiation to the sample and creates the evanescent wave used for measurement. Because this field extends only a short distance beyond the element, the resulting signal emphasizes molecular groups near the contacted region, enabling analysis with limited sample disruption.
Interpretation links absorption features to molecular vibrational modes. Distinct portions of a spectrum can therefore indicate functional groups associated with proteins, lipids, polymers, or other constituents. Comparing those features across samples helps determine whether composition or molecular structure has changed, rather than treating the spectrum as an isolated identification label.
The short penetration depth makes the measurement especially useful when changes occur at or near the analyzed surface or interface. In biological and engineered materials, ATR-FTIR can examine a sample while limiting the volume interrogated by the infrared field. This supports minimally destructive comparisons of localized chemical composition and structure.
A basic workflow is to position the material or biological sample against the internal reflection element, collect its infrared spectrum, and compare the resulting absorption pattern with spectra from relevant samples or conditions. Because the method generally requires little sample preparation, it can support characterization of proteins, lipids, polymers, biomaterials, or cell-associated changes.
The technique can characterize diverse bioengineering materials and biological constituents, including proteins, lipids, polymers, biomaterials, and cell-associated chemical changes. This range allows one measurement approach to support investigations of both biological composition and engineered material structure, making it relevant across tissue engineering, drug delivery, and biomedical device development.
Spectral comparisons reveal differences in functional groups, chemical composition, or molecular structure between samples. In bioengineering, researchers can use these changes to assess biochemical interactions, monitor cell-associated or material-related transformations, and evaluate consistency during development. The same comparison-based approach also supports quality control for tissue-engineering materials, drug-delivery systems, and biomedical devices.