Dna Nanoparticle Transfection
Dna Nanoparticle Transfection - Related Videos
08:35
Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
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Cited by 28 •
2012
Type B gelatin-based engineered nanovectors system (GENS) was developed for systemic gene delivery and transfection in the treatment of pancreatic cancer. By modification with epidermal growth factor receptor (EGFR) specific peptide on the surface of nanparticles, they could target on EGFR receptor and release plasmid under reducing environment, such as high intracellular glutathione concentrations.
15:56
DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
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Cited by 99 •
2009
We describe detailed procedures for the efficient transfection of plasmid DNA into the fibers of foot muscles of live mice using electroporation and the subsequent visualization of protein expression using fluorescence microscopy.
05:11
Organoid Electroporation: A Method to Transfect Plasmid DNA into Gastrointestinal Organoids
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2023
This video describes the electroporation protocol for transfecting large plasmids into three-dimensional gastrointestinal organoids to prove its universal functionality in different entities, namely pancreatic ductal adenocarcinoma (PDAC), colorectal cancer (CRC), cholangiocarcinoma (CCC), and gastric cancer (GC) organoids.
07:28
An Introduction to Transfection
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2023
Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA into a cell enables the expression, or production, of proteins using the cells own machinery, whereas insertion of RNA into a cell is used to down-regulate the production of a specific protein by stopping translation. While the site of action for transfected RNA is the cytoplasm, DNA must be transported to the nucleus for effective transfection. There, the...
03:43
Magnetofection-Based Transfection In Vitro: A Magnetic Field-Assisted Technique to Deliver Plasmids Into Primary Mouse Neuronal Cells Using Magnetic Nanoparticles
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2025
In this video, we demonstrate magnetically-guided transfection of plasmid DNA in primary neuronal cell culture. Magnetofection uses an external magnetic field to guide the delivery of plasmids bound to magnetic nanoparticles into cell cytoplasm.
FAQs
After exposure, cells often internalize the DNA-containing particles through endocytosis, a process in which the cell membrane encloses material in an internal compartment. Subsequent intracellular processing must release the DNA cargo from the nanoparticle and support its movement to the nucleus. Once nuclear access occurs, the construct can be transcribed, linking particle behavior to the resulting gene-expression pattern.
Particle size, surface chemistry, cargo loading, and exposure conditions can alter how efficiently cells take up the particles and how well they support intracellular DNA release. These variables also affect cell compatibility and the duration of gene expression. In bioengineering experiments, systematic tuning helps researchers distinguish whether limited expression reflects uptake, processing, or downstream access to the nucleus.
The approach uses nanoparticle materials rather than a viral vector to assemble with and protect plasmid DNA or another DNA construct. This distinction matters when researchers seek a nonviral option while still requiring cellular uptake, intracellular processing, and nuclear transport. Its performance is evaluated through the balance among transfection efficiency, cell compatibility, and the duration of gene expression.
A typical workflow begins by selecting the DNA cargo and nanoparticle material, then assembling them into particles with an intended size, surface chemistry, and cargo loading. The particles are exposed to cells under defined conditions. Researchers then consider uptake, intracellular DNA release, nuclear transport, and transcription, while assessing efficiency, compatibility, and expression duration.
Optimization requires balancing delivery performance with cellular compatibility rather than maximizing uptake alone. Adjusting particle size, surface chemistry, cargo loading, or exposure conditions can change transfection efficiency and cell compatibility. The preferred formulation also depends on whether the experiment needs short-term or more sustained gene expression, so outcomes should be interpreted together rather than through a single performance measure.
In bioengineering, the method can support gene-function studies by introducing DNA constructs for controlled cellular gene expression. It also contributes to engineered-cell research and tissue-regeneration studies, where regulated expression is relevant. The same delivery strategy has potential for therapeutic delivery, making particle design and expression duration important when moving from exploratory experiments toward biomedical applications.