Ductal Puncture

Ductal puncture is a minimally invasive technique that uses a fine needle to access the lumen of a biological duct for sampling, fluid delivery, or measurement. The needle is carefully advanced through surrounding tissue into the duct, where controlled insertion and pressure management help limit leakage, obstruction, and tissue damage; the procedure may be guided by anatomical or imaging information. In bioengineering, ductal puncture supports the study of transport pathways, fluid mechanics, and tissue-device interactions, while enabling localized delivery and collection of biological materials. These applications can inform diagnostic tools, engineered tissue models, and minimally invasive therapeutic systems.

Ductal Puncture - Related Videos

Research

JoVE EoE - Pancreatic Cancer

Acinar-to-Ductal Metaplasia Induction: A Method to Study Acinar Cell to Ductal Cell Differentiation in 3D Ex Vivo Culture

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2023

This video describes the technique of differentiating pancreatic acinar cells to ductal cells in ex vivo 3D culture. This helps to understand the mechanisms regulating the acinar-to-ductal metaplasia (ADM), a key process in the early development of pancreatic cancer.

Cecal Ligation Puncture Procedure

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Cited by 200 •

2011

The mouse model of cecal ligation and puncture as a valuable tool for the study of human sepsis.

Cardiac Blood Collection via Subclavian Puncture in Mice

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2025

Subclavian cardiac puncture is a simple, reliable method for collecting terminal blood in mice. It is easy to operate and ensures high-purity samples.

Ultrasound-Guided Implantation: A Technique to Implant Pancreatic Ductal Adenocarcinoma Cells into an Adult Mouse

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2023

This video describes the protocol for ultrasound-guided orthotopic implantation of murine-derived pancreatic ductal adenocarcinoma cells directly into the pancreas of the mouse. This causes less inflammation at the implantation site compared to surgical procedures.

Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture

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Cited by 87 •

2007

Blood draws are necessary in a large number of studies, for example to study the pharmacokinetics profile of a compound. Here, we demonstrate how to draw blood from rats using two techniques: blood draw from the saphenous vein or by cardiac puncture.

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