Phosphor Imaging
Phosphor Imaging - Related Videos
07:03
Low-energy Cathodoluminescence for (Oxy)Nitride Phosphors
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Cited by 2 •
2016
An excellent chemical and luminescence stabilities of (oxy)nitride phosphors present it as an promising alternative to currently used sulfide and oxide phosphors. In this paper, we present the way to investigate its local luminescence properties using low-energy cathodoluminescence (CL).
06:16
Phase-Dependent Control of Trap Depth and Persistent Luminescence in Strontium Aluminate Phosphors
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2025
This study investigates the standard optical measurements of two persistent luminescent ceramics: SAO-B (blue) and SAO-G (green), such as thermoluminescence (TL) and persistent luminescence (PersL). By controlling electron trap depth, this work innovates 'temperature-resolved' and 'time-resolved' dynamic anti-counterfeiting methods.
13:45
Hybrid µCT-FMT imaging and image analysis
0 Views •
Cited by 34 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
FAQs
Signal formation depends on two linked events. During autoradiography, radiation from a labeled biological sample deposits energy in a storage phosphor screen. A laser scanner then stimulates the screen, causing it to emit light. The emitted light is converted into a digital signal, which becomes an image suitable for research analysis.
The storage phosphor screen serves as the intermediate surface that captures energy emitted by radioisotopes during autoradiography. It preserves the recorded radiation pattern until laser stimulation releases it as light. This function connects the labeled sample with the scanner, allowing the spatial distribution of radioactive signal to be converted into a digital image.
A broad range of signal intensities allows phosphor imaging to represent differences in radiation recorded from labeled biological material. Its digital output supports image-based measurement of radiolabeled DNA, RNA, proteins, and other molecules. Consequently, the same analysis can provide both visualization and quantitative information about the detected sample.
A typical workflow places the radiolabeled biological sample in an autoradiographic setup with a storage phosphor screen. The screen captures energy emitted by the radioisotope, after which a laser scanner stimulates the stored signal. The resulting light is converted into a digital signal and image for subsequent research analysis and measurement.
Phosphor imaging can be applied to radiolabeled DNA, RNA, proteins, and other molecules present in gels, blots, or tissue sections. The suitable format depends on where the labeled material is being studied. This range enables researchers to examine molecular signals in separated samples as well as in tissue-based distributions.
Researchers use phosphor imaging to investigate gene expression, molecular interactions, and the distribution of biomolecules. In biological techniques, it is useful when a study requires an image of radiolabeled material together with quantitative measurement. Applications can therefore span gel- and blot-based analyses as well as examination of labeled molecules within tissue sections.