Phosphatidylserine
Phosphatidylserine - Related Videos
04:21
Annexin V Binding Assay: A Fluorescence-Based Technique to Identify Apoptotic Erythrocytes via Phosphatidylserine Labeling
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2025
In this video, we demonstrate the Annexin V binding assay to measure the degree of eryptosis, erythrocyte programmed cell death, using fluorescent Annexin V staining. Apoptotic erythrocytes demonstrate phosphatidylserine translocation from the inner to the outer leaflet of the cell membrane, causing the fluorophore-tagged Annexin V to bind to the translocated phosphatidylserine, thereby emitting a fluorescent signal.
08:49
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
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Cited by 1 •
2021
Here, we describe protocols using fluorescent lipid sensors and liposomes to determine whether a protein extracts and transports phosphatidylserine or phosphatidylinositol 4-phosphate in vitro.
05:49
Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
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Cited by 2 •
2024
Procoagulant platelet formation has been correlated with an increased risk of thrombosis. Presented here is a precise protocol for isolating washed platelets from human blood, intended to quantify the exposure of phosphatidylserine and microvesicle release, which are distinctive features of procoagulant platelets.
03:33
In Vitro Phagocytosis Assay: A Live Imaging Based Method to Study Real-time Astrocyte Mediated Synaptosome Phagocytosis
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2025
In this video, we evaluate the phagocytic capacity of purified astrocytes in vitro by live imaging assay. The assay serves as an effective screening platform to identify chemical compounds that can enhance or inhibit the phagocytic capacity of astrocytes.
03:13
An In Vitro Assay to Assess CAR T Cell Cytotoxicity against Tumor Spheroids
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2025
This video demonstrates an in vitro assay evaluating the cytotoxic effects of CAR T cells on 3D tumor spheroids expressing fluorescent proteins. This assessment utilizes the Annexin V red fluorescent probe, which binds to exposed phosphatidylserine residues on the outer leaflet of apoptotic cancer cells after interactions with CAR T cells. The red fluorescence emitted by the probe aids in distinguishing these cells from healthy ones emitting green fluorescence.
FAQs
Lipid transport enzymes help maintain phosphatidylserine predominantly on the membrane’s inner leaflet rather than allowing an even distribution between the two sides. This controlled asymmetry is biologically important because changing the lipid’s location alters the information presented at the cell surface. Studying these transport processes helps explain how membrane organization is actively regulated.
During apoptosis, phosphatidylserine moves to the outer membrane surface, where it functions as an “eat-me” signal. This exposure allows phagocytes to recognize cells that are undergoing programmed death and supports their removal. The process links a change in membrane lipid distribution with orderly cellular clearance rather than leaving dying cells unrecognized.
Phosphatidylserine provides a negatively charged membrane surface that supports the assembly of enzyme complexes involved in blood coagulation. Its contribution is therefore functional, not merely structural: the lipid’s charge and location help create a membrane environment relevant to hemostasis. Research on this role connects membrane biology with the mechanisms that regulate clot formation.
Redistribution changes which membrane surface contains an anionic, or negatively charged, lipid and can consequently alter cellular signaling conditions. Because phosphatidylserine also contributes to membrane structure, its movement connects physical membrane organization with biological communication. This makes lipid asymmetry an important variable when researchers examine how cells respond to internal changes such as apoptosis.
Studies of phosphatidylserine can address how eukaryotic cells organize their membranes, regulate programmed cell death, and communicate with phagocytes during immune clearance. The same research area also examines how membrane surfaces support blood coagulation. Together, these applications make the lipid relevant to cell biology, immunology, hemostasis, and investigations of related biological disorders.
Its relevance comes from the connection between lipid redistribution and the removal of dying cells. When apoptosis exposes phosphatidylserine externally, phagocytes can identify the affected cell through the resulting “eat-me” signal. Researchers can therefore use this membrane event to investigate how programmed cell death becomes linked to recognition and clearance by other cells.
Phosphatidylserine research connects membrane organization with two major cellular outcomes: immune removal of apoptotic cells and formation of coagulation-supporting surfaces. These links show how one membrane component can participate in signaling, cellular disposal, and hemostasis. In biology, examining this lipid provides a way to study interactions between cell membranes, immune processes, and blood-related functions.