Angiogenic Response
Angiogenic Response - Related Videos
11:07
In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
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Cited by 3 •
2019
The aim of this method is to generate an in vivo model of tumor angiogenesis by xenografting mammalian tumor cells into a zebrafish embryo that has fluorescently-labelled blood vessels. By imaging the xenograft and associated vessels, a quantitative measurement of the angiogenic response can be obtained.
10:04
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
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Cited by 12 •
2017
Here, we present a novel application of the aortic ring assay where prelabelled mesenchymal cells are co-cultured with rat aorta-derived endothelial networks. This novel method allows visualization of Mesenchymal Stromal Cells (MSCs) homing and integration with endothelial networks, quantification of network properties, and evaluation of MSC immunophenotypes and gene expression.
04:21
Generating Anti-Angiogenic Tumor-Associated Neutrophils
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2025
This video demonstrates an assay to generate anti-angiogenic tumor-associated neutrophils (TANs). Upon taking a murine melanoma tumor containing pro-angiogenic TANs, the single cells are isolated using fluorescence-activated cell sorting (FACS) and treated with an inhibitor of nicotinamide phosphoribosyl transferase (NAMPT) to reduce the capacity to stimulate angiogenesis.
08:19
Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
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Cited by 5 •
2019
Here, we show therapeutic potential of anti-angiogenic tumor-associated neutrophils after their transfer into tumor-bearing mice. This protocol can be used to manipulate neutrophil activity ex vivo and to subsequently evaluate their functionality in vivo in developing tumors. It is an appropriate model for studying potential neutrophil-based immunotherapies.
05:22
A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
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2025
Multiplex fluorescence immunoassays are becoming a reference for serology testing. Their application, however, is limited by the complexity and time-consuming bead-analyte coupling processes. This report presents a semi-automated, reproducible multiplex microsphere bead coupling protocol that is comparable to the standard method while considerably reducing time to results.
FAQs
Low oxygen can act as an initiating condition for the angiogenic response, prompting tissue-derived signaling that includes vascular endothelial growth factor, or VEGF. These cues guide endothelial cells toward the affected region, where basement membrane breakdown permits movement out of existing vessels. This sequence links local oxygen status to directed vascular expansion during development and repair.
Basement membrane degradation creates a path for endothelial cells to leave the existing vessel and migrate toward tissue-derived signals. Without this structural change, cells could not readily extend sprouts into surrounding tissue. Its position early in the sequence helps coordinate directed movement with later endothelial proliferation, branching, lumen formation, and vessel stabilization.
Endothelial cells first migrate toward the relevant signal and proliferate, producing sprouts that extend through developing tissue. Continued branching increases the vascular pattern, while lumen formation creates internal vessel spaces. Stabilization then helps mature the new structures. Together, these stages convert a local developmental cue into an organized network able to support tissue needs.
Insufficient angiogenesis can limit the vascular support needed for tissue growth, repair, or regeneration, whereas abnormal angiogenesis can disrupt normal development and tissue patterning. The outcome depends on how effectively signaling, endothelial movement, proliferation, branching, and maturation are coordinated. Examining these failures helps connect vascular behavior with developmental and disease-related tissue changes.
A useful analysis follows the response across several observable stages: basement membrane degradation, endothelial migration toward a signal, cell proliferation, branching, lumen formation, and stabilization. Considering the sequence rather than a single event reveals whether vascular development is progressing normally. These features provide a framework for relating vascular structure to oxygen supply, tissue growth, and repair.
During development, coordinated vessel formation supplies oxygen and nutrients while helping shape organs and tissue patterns. The same principles are relevant to wound healing and regeneration, where vascular support affects repair. In tissue engineering, understanding these processes can inform strategies intended to support vascularized tissues, while developmental studies provide context for vascular disease.