Anti-infective Evaluation
Anti-infective Evaluation - Related Videos
06:56
An Assay for Quantifying Antibody-Mediated Phagocytosis of Parasite-Infected Erythrocytes
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2025
This video demonstrates a flow cytometry-based phagocytosis assay involving labeled and opsonized Plasmodium falciparum-infected erythrocytes by monocytes. Flow cytometry identifies fluorescence in monocytes, confirming the successful identification of opsonized erythrocytes through phagocytosis.
05:35
An In Vitro Assay to Examine the Antibody-Dependent Enhancement of Viral Infection
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2025
This video demonstrates an assay to measure antibody-dependent enhancement (ADE) of Dengue virus infection using Zika Virus Convalescent Serum. The assay uses Dengue reporter viral particles (RVPs) encoding a green fluorescent protein. Serum antibodies against the Zika Virus cross-react with RVPs to form a complex. The bound antibodies enhance RVP uptake by cells expressing the Fc receptor, which is detected using flow cytometry.
10:25
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
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2021
The protocol described here outlines a fast and effective method for measuring neutralizing antibodies against the SARS-CoV-2 spike protein by evaluating the ability of convalescent serum samples to inhibit infection by an enhanced green fluorescent protein-labeled vesicular stomatitis virus pseudotyped with spike glycoprotein.
04:59
Evaluating the Intracellular Distribution of Virus-Derived Peptide-Modified Antibody Conjugates
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2025
This video demonstrates the process of assessing the intracellular distribution of virus-derived peptide-modified antibody conjugates using confocal microscopy. The viral-derived nuclear localization sequence directs antibody-conjugates within the cytoplasm, confirmed through immunostaining and fluorescence measurement.
03:54
An In Vitro Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilm
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2025
This video demonstrates an in vitro technique to study the effect of antibodies on fungal biofilms. The pathogenic fungus Candida tropicalis secretes a virulence factor secreted aspartyl proteinase 2 (Sap2), facilitating biofilm formation. Upon introducing a heat-inactivated serum containing Sap2-specific antibodies that inhibit biofilm maturation, the viability of the fungal cells is assessed using a chromogenic assay.
FAQs
Anti-infective evaluation can distinguish antimicrobial potency, selectivity, and potential resistance development rather than treating these outcomes as interchangeable. Potency indicates how strongly a candidate affects a target microorganism or infected cell. Selectivity considers the treatment’s activity in relation to its effects on host cells. Tracking resistance development adds a longer-term dimension to judging whether an intervention could remain useful.
The target determines which biological response the study can examine. Researchers may expose microorganisms directly to a candidate treatment or evaluate infected cells, allowing assessment of microbial growth, viability, or replication alongside host-cell responses. This distinction helps separate effects on the infectious agent from effects associated with the infected host environment and supports more focused interpretation of treatment activity.
Immune-based interventions can be assessed by examining how they influence infected cells, host-cell responses, and interactions between the host and pathogen. Their evaluation complements direct measurements of microbial growth, viability, or replication because an intervention may be understood through both infectious-agent outcomes and immune-related effects. This combined perspective supports investigation of host-pathogen interactions and biologic development.
A laboratory evaluation begins by selecting a microorganism or infected-cell system and a compound, therapy, or immune-based intervention for assessment. Researchers then expose the target to the candidate under defined conditions and measure relevant outcomes, such as microbial growth, viability, replication, or host-cell responses. Comparisons among candidates can reveal differences in activity, selectivity, and resistance development.
The most informative outcomes depend on the intervention and experimental target, but the source identifies microbial growth, viability, replication, and host-cell responses as central measurements. Examining these results together can show whether a candidate affects the infectious agent, the infected cellular environment, or both. Such measurements also support comparisons of antimicrobial potency and selectivity under defined conditions.
This approach supports several research decisions, including drug discovery, therapeutic selection, vaccine and biologic development, and studies of host-pathogen interactions. Results can help compare candidate interventions before further development and clarify how they interact with pathogens or the immune system. Ultimately, the findings contribute to translating laboratory observations into safer and more effective infection-control strategies.