Endocytic Potential Assessment
Endocytic Potential Assessment - Related Videos
08:02
Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells
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Cited by 1 •
2025
Endocytic and retrograde transport of proteins from the plasma membrane to the trans-Golgi network is essential to maintaining membrane homeostasis and regulating signaling. Here, we describe a method to image and quantify endocytic transport of transmembrane cargo proteins by live-cell microscopy using derivatized anti-GFP nanobodies in HeLa cells.
11:32
Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
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Cited by 23 •
2009
Regulated endocytosis governs the cell surface expression levels of the majority of membrane proteins. Here we utilize reducible, membrane impermeant biotinylation reagents to measure the endocytic rate of the dopamine transporter (DAT), a polytopic membrane protein. The method facilitates a straightforward approach to measuring the endocytic rate of most plasma membrane proteins.
07:29
Zebrafish as a Model to Assess the Teratogenic Potential of Nitrite
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Cited by 17 •
2016
Exposure to teratogens may cause birth defects. Zebrafish are useful for determining the teratogenic potential of chemicals. We demonstrate the utility of zebrafish by exposing embryos to various levels of nitrite and also at different times of exposure. We show that nitrite can be toxic and cause severe developmental defects.
08:29
Standardized Induction and Assessment of Long-term Potentiation-like Cortical Plasticity Using Transcranial Magnetic Stimulation
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2025
This paper describes the standardized induction of Long-term Potentiation-like cortical plasticity using repetitive stimulation protocols, followed by applying single-pulse transcranial magnetic stimulation guided by a neuronavigation system to evaluate synaptic plasticity.
02:49
Assessing the Antimicrobial Potential of Bacteriocin-Producing Murine Fecal Bacterial Isolates
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2025
This video demonstrates the detection of bacteriocin-producing lactic acid bacteria derived from mouse fecal matter. The assay determines the antimicrobial activity of bacteriocin on specific bacterial strains.
FAQs
The key analytical distinction is whether labeled cargo remains at the plasma membrane or becomes enclosed in endocytic vesicles. Fluorescence imaging can localize the signal, whereas flow cytometry can quantify cell-associated fluorescence when the assay distinguishes surface-bound from internalized material. This prevents membrane attachment from being interpreted as uptake and makes comparisons of cellular capacity more meaningful.
Cargo selection helps align the measurement with the biological question. A labeled particle, protein, or microorganism places the assay in a particular uptake context, while endocytosis itself contributes to nutrient uptake, receptor signaling, antigen processing, and infection. Results should therefore be interpreted in relation to the selected cargo and cellular response, rather than treated as a context-free measure.
Endocytic Potential Assessment can expose treatment-dependent changes in entry pathways. Investigators compare the amount of labeled material internalized by cells under the relevant conditions, while retaining the distinction between surface-associated and enclosed cargo. A change in the internalization readout helps indicate that treatment affected cellular entry behavior, rather than merely changing how much material was attached to the cell exterior.
A basic workflow begins by presenting cells with a labeled cargo, such as a particle, protein, or microorganism. The assay then measures associated fluorescence with imaging or flow cytometry and distinguishes material on the cell surface from material enclosed in endocytic vesicles. This sequence produces an uptake measurement that can be compared across cells or experimental conditions.
Fluorescence imaging and flow cytometry provide complementary ways to quantify the assay signal. Imaging helps determine where labeled cargo is located, including whether it is enclosed in vesicles, while flow cytometry supports fluorescence-based quantification across analyzed cells. The choice depends on whether the study emphasizes intracellular localization, comparative uptake, or both.
In immunology, the measurement can compare uptake by cells involved in antigen presentation and examine consequences for antigen processing. In infection research, it supports analysis of microbial entry and host-pathogen interactions. These applications connect a cellular fluorescence readout with broader questions about immune surveillance, cellular entry, and mechanisms of microbial invasion.