Murine Islet Culture
Murine Islet Culture - Related Videos
15:20
Murine Pancreatic Islet Isolation
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Cited by 119 •
2007
03:48
Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding
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2025
In this video, we describe a procedure to infuse pancreatic islet cells into the hepatic portal vein, followed by application of gel foam to successfully prevent bleeding. Postoperative bleeding is a common cause of high mortality during islet transplantation.
17:42
A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
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Cited by 134 •
2011
Transplantation of isolated islets has been proposed to be a potential treatment for type 1 diabetes. Here we describe a method to isolate islets from mouse pancreata and transplant them to the subcapsular space of the kidney.
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
09:41
Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets
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Cited by 12 •
2017
We describe a protocol for visualization of insulin exocytosis in intact islets using pHluorin, a pH-sensitive green fluorescent protein. Isolated islets are infected with adenovirus encoding pHluorin coupled to the vesicle cargo neuropeptide Y. This allows for the detection of insulin granule fusion events by confocal microscopy.
FAQs
Maintaining intact islets preserves coordinated interactions among beta, alpha, and other endocrine cells. This organization allows investigators to examine endocrine function as a multicellular response rather than measuring beta cells in isolation. Consequently, experiments can assess how signals regulating blood-glucose homeostasis influence the integrated behavior of several pancreatic endocrine populations.
Collagenase digestion helps release pancreatic islets from surrounding tissue by breaking down components of the pancreatic structure. The resulting preparation can then be separated from exocrine material before culture. This separation is important because the experimental model is intended to evaluate endocrine islet responses, including insulin secretion and cellular viability, under defined conditions.
Functional maintenance depends on nutrient-defined culture medium together with controlled temperature and carbon dioxide conditions. These parameters provide a stable environment in which islets can remain viable and respond to experimental stimuli. Consistent control is especially important when comparing glucose-stimulated insulin secretion or evaluating changes caused by hormones, inflammatory signals, or candidate therapies.
Glucose stimulation provides a controlled challenge for measuring insulin secretion from cultured islets. The response can indicate how effectively endocrine tissue detects and reacts to glucose, making it useful for studying mechanisms related to blood-glucose regulation. Comparing responses across treatments or disease-relevant conditions can help identify altered endocrine function or potential therapeutic effects.
A typical preparation begins with pancreatic tissue digestion using collagenase, followed by separation of islets from exocrine material. The recovered islets are then maintained in nutrient-defined medium under controlled temperature and carbon dioxide conditions. Once cultured, they can be exposed to glucose, hormones, inflammatory signals, or candidate therapies and assessed for functional or viability-related outcomes.
This model supports measurement of glucose-stimulated insulin secretion and assessment of beta-cell viability. Investigators can also examine how cultured islets respond to hormones, inflammatory signals, or candidate therapies. Together, these outcomes connect cellular health with endocrine performance, helping distinguish an intervention that preserves viability from one that directly changes secretory function.
Cultured mouse islets provide a controlled setting for investigating diabetes pathophysiology, mechanisms that regulate blood-glucose homeostasis, and responses to potential treatments. Their preserved interactions among multiple endocrine cell types add biological context to secretion and viability studies. This makes the system useful for evaluating drug efficacy while examining how disease-related or inflammatory signals affect endocrine function.