Hcr-fish
Hcr-fish - Related Videos
06:00
HCR-DNA FISH: A Fluorescence In Situ Hybridization Technique to Detect Viral DNA in Infected Cells
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2023
In this video, the cells infected with MCPyV virions were subjected to in situ hybridization chain reaction to detect MCPyV specific genomes. Further, the DNA-HCR technique was combined with FISH to visualize the MCPyV DNA in infected human skin cells.
07:25
Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria
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Cited by 2 •
2017
A series of basic methods to enable the study of the reproductive ecology of fish kept in aquaria are described. These are useful protocols for collecting fish using SCUBA, transporting live fish, and observing the reproductive behavior of wild-caught fish kept in aquaria.
02:32
Osmoregulation in Fishes
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2019
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments? Fish employ osmoregulatory strategies to balance bodily levels of water and dissolved ions (i.e., solutes), such as sodium and chloride. Imagine two solutions separated by a membrane that is permeable to water.
05:12
Swimming Performance Assessment in Fishes
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Cited by 56 •
2011
The lives of the majority of fish are predicated on swimming. This protocol describes techniques for capturing a range of swimming modes available to individual and schooling fish, and includes metrics associated with swimming physiology and behaviour.
07:57
Necropsy-based Wild Fish Health Assessment
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Cited by 21 •
2018
The health of wild fishes can be used as an indicator of aquatic ecosystem health. Necropsy-based fish health assessments provide documentation of visible lesions or abnormalities, data used to calculate condition indices as well as the opportunity to collect tissues for microscopic evaluation, gene expression and other more in-depth analyses.
FAQs
Target-specific probes first bind complementary nucleic acid sequences inside the sample. This binding creates a site that activates fluorophore-labeled hairpins. The hairpins alternately open and polymerize into a chain, concentrating many fluorescent labels at the original target location. Because assembly occurs through probe-triggered hairpin interactions, the signal is amplified without enzymatic amplification.
HCR-FISH generates amplified fluorescence through self-assembling hairpins rather than an enzyme-driven amplification reaction. This mechanism supports detection while the target remains in intact cells and tissues, allowing researchers to relate transcript-associated signals to neuronal structure, brain-region organization, and other anatomical features that might be difficult to interpret without preserved spatial context.
Researchers can use multiplexed probe sets to detect multiple transcripts in the same neural sample. Each target-specific probe set identifies a selected nucleic acid sequence, while the resulting fluorescent signals reveal where those targets occur relative to one another. This makes it possible to examine spatial relationships among transcripts rather than analyzing each gene-expression pattern in isolation.
The method preserves cellular and anatomical context while mapping nucleic acid signals across neurons, brain regions, and other neural tissues. Consequently, researchers can connect gene-expression patterns with the locations of cells and structures in the nervous system. This spatial organization helps interpret molecular differences in relation to neural development, cell identity, connectivity, or disease-associated changes.
A typical workflow begins with target-specific probes binding selected RNA or DNA sequences in intact cells or tissues. Fluorophore-labeled hairpins are then introduced so probe-bound sites trigger alternating hairpin opening and polymerization. The assembled fluorescent chains produce localized signals that can be imaged and compared across cells, anatomical regions, or multiplexed transcript targets.
HCR-FISH is useful when a study needs both gene-expression information and the position of that expression within neural tissue. Applications include mapping transcripts during neural development, distinguishing cell identities, examining connectivity-related molecular patterns, and investigating disease-associated changes. Its value is greatest when spatial relationships among cells, regions, or multiple transcripts are scientifically important.
Images can show the presence and localization of selected RNA or DNA sequences within intact neural samples. With multiplexed probe sets, investigators can compare transcript distributions and identify spatial relationships among molecular signals. These observations support analyses of gene expression across neurons and brain regions, helping connect molecular patterns with neural organization and disease-related changes.