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Visualisera Live Drosophila Gliaceller-neuromuskulära förbindelsen med fluorescerande färger

Article DOI: 10.3791/1154-v • 10:53 min • May 13th, 2009
May 13th, 2009 •
1, 1, 1
1Department of Zoology, University of British Columbia - UBC

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Vi beskrev strukturella drag hos Glia-neuromuskulära synapser i en roman Inifrån-ut vävnad förberedelse av levande fluglarver med fluorescerande färger med konfokalmikroskopi. Vi märkta lever nervterminaler med fluorescerande primära antikroppar mot HRP och även visualiseras i perisynaptic utrymmet med fluorescerande dextraner.

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Neurovetenskap Drosophila fluorescens Glia NMJ synapsen Bildproduktion preparat permeationskvot
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