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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3
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April 13, 2009
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Protocol for Vaccinia infection of HeLa cells and analysis of host and viral gene expression. Part 3 describes the process of fluorescently labeling the amplified RNA from both host and viral samples by amino allyl coupling of dyes. Part 3 of 3.
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Vaccinia Virus InfectionTemporal AnalysisVirus Gene ExpressionPoxviridae FamilyOrthopox GenusVariolaSmall PoxMonkeypoxVaccinia (VAC)Virus GenomeEarly GenesIntermediate GenesLate GenesViral RNA PolymerasesHost Cellular Environment ModulationTranscript Abundance AnalysisHeLa CellsTime Course InfectionsRNA IsolationRNA AmplificationMicroarray AnalysisGene Expression
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Yen, J., Golan, R., Rubins, K. Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3. J. Vis. Exp. (26), e1170, doi:10.3791/1170 (2009).
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