Inferenza genotipica del virus HIV-1 Tropismo utilizzo basati sulla popolazione sequenziamento del V3
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December 27, 2010
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Tropismo dell'HIV può essere dedotta dalla regione V3 della busta virale. V3 è amplificato mediante PCR in triplice copia tramite nested RT-PCR, in sequenza, e interpretato utilizzando il software bioinformatico. I campioni con con sequenza di 1 o più (s) con punteggi bassi G2P sono classificati come non-R5 virus.
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McGovern, R. A., Harrigan, P. R., Swenson, L. C. Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3. J. Vis. Exp. (46), e2531, doi:10.3791/2531 (2010).
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