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Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology

Article DOI: 10.3791/2533-v • 12:50 min • March 31st, 2011
March 31st, 2011 •
1, 1, 1,2
1Department of Biology, Syracuse University, 2Department of Science Teaching, Syracuse University

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Whole mount in situ hybridization (WISH) was used in an upper level undergraduate Comparative Vertebrate Biology course in addition to vertebrate dissections. This gave students the opportunity to study gene expression patterns as well as gross anatomy, linking the study of molecular and organismal biology within one course.

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Whole Mount In Situ Hybridization Gene Expression MRNA Transcripts Molecular Biology Organismal Biology Comparative Vertebrate Biology Lab Embryology Gross Anatomy Dissections Models Vertebrate Development Zebrafish Embryos Fibroblast Growth Factor 8 A (fgf8a) Mutant Line RNA Probes Midline Expression Heart Development Somites Tailbud Myotome Brain Development Staining Reaction
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