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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
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Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays

Article DOI: 10.3791/2784-v • 13:12 min • April 4th, 2011
April 4th, 2011 •
*1, *2, 2, 1,3,4, 1, 5, 5, 1,3
1Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB, Health canada, 2National Institute for the Control of Pharmaceutical and Biological Products, The State Food and Drug Administration, Beijing, 3Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 4Microbiology Department, Faculty of Medicine, King Abdulaziz University, 5National Microbiology Laboratory, Public Health Agency of Canada
* These authors contributed equally

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A simple slot blot method was developed for the quantification of influenza viral hemagglutinin and neuraminidase using universal antibodies targeting their most conserved sequences identified through bioinformatics analyses. This innovative approach may provide a useful alternative to quantitative determination of all viral hemagglutinin and neuraminidase.

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Quantitative Analyses Influenza Type A Viral Hemagglutinins Neuraminidases Universal Antibodies Simple Slot Blot Assays Surface Proteins Viral Life Cycle Protective Immune Responses Neutralizing Antibodies Influenza Vaccine Potency Marker Single Radial Immunodiffusion (SRID) Subtype-specific Antisera New Vaccine Release NA Antigens Reagents Analytical Method Universally Conserved Regions Bioinformatics Analyses Fusion Peptide Enzymatic Active Site N-terminus
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