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A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Article DOI: 10.3791/4041-v • 07:10 min • July 31st, 2012
July 31st, 2012 •
*1, *1, *1, 1
1Department of Laboratory Medicine and Pathobiology, University of Toronto
* These authors contributed equally

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In the post-human genomics era, the availability of recombinant proteins in native conformations is crucial to structural, functional and therapeutic research and development. Here, we describe a test- and large-scale protein expression system in human embryonic kidney 293T cells that can be used to produce a variety of recombinant proteins.

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Convenient Expression Platform Secreted Proteins Recombinant Protein Expression Human Cells Bacteria E. Coli Prokaryotic Hosts Human Proteins Yeast Expression System Baculovirus-infected Insect Cells Cell-free In Vitro Translation System Mammalian Host Mammalian Cell Lines HEK 293 Cells CV-1 Cells CHO Cells Protein Yields Cost-effective Production
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