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Creating Defined Gaseous Environments to Study the Effects of Hypoxia on C. elegans

Article DOI: 10.3791/4088-v • 11:07 min • July 20th, 2012
July 20th, 2012 •
1,2, 1, 1
1Department of Biochemistry, University of Washington, 2Molecular and Cellular Biology Program, University of Washington

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This paper details how to use continuous-flow hypoxia chambers to generate atmospheres with defined concentrations of O2 to understand biological responses to decreased O2. This system is easy to setup and maintain, and flexible enough to suit a wide range of O2 concentrations and model systems

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Defined Gaseous Environments Hypoxia C. Elegans Cellular Signaling Pathways Treatment Strategies Model Organism Genetically Amenable Hypoxic Response O2 Concentrations Transcriptional Response Hif-1 Ppm O2
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