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Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Article DOI: 10.3791/4108-v • 08:37 min • August 7th, 2012
August 7th, 2012 •
1, 2,3, 2,3,4
1Sanford-Burnham Medical Research Institute, 2Division of Dermatology, University of California, San Diego, 3VA San Diego Healthcare Center, 4Moores Cancer Center, University of California, San Diego

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Considering saliva sampling for future clinical application, a lollipop-like ultrafiltration (LLUF) probe was fabricated to fit in the human oral cavity. Direct analysis of undigested saliva by NanoLC-LTQ mass spectrometry demonstrated the ability of LLUF probes to remove large proteins and high abundance proteins, and make low-abundant peptides more detectable.

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Human Indigenous Saliva Peptidome Lollipop-like Ultrafiltration Probe Peptide Detection Clinical Mass Spectrometry Saliva Proteome Tryptic Digests Exogenous Enzymes Native Peptides Disease Diagnosis Disease Progression Prediction Therapeutic Efficacy Monitoring Sampling Methods Debris Removal Infected Pathogens High Abundance Proteins Low Abundance Peptidome Conventional Proteomic Approaches Two-dimensional Gel Electrophoresis (2-DE) In-gel Digestion Liquid Chromatography-Mass Spectrometry (LC-MS)
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