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JoVE Journal Biology
A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants
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A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

Article DOI: 10.3791/4368-v • 06:38 min • May 1st, 2013
May 1st, 2013 •
1, 2, 3, 1
1Department of Experimental Oncology, European Institute of Oncology, 2Department of Pathology and Laboratory Medicine, European Institute of Oncology, 3U.O. Gastroenterologia 2, IRCCS Ca' Granda, Ospedale Policlinico di Milano

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We introduce a novel method for the maintenance of human intestinal mucosa in culture and monitoring of the response to various types of stimuli over at least 24 hrs. With our method, the polarity of the tissue is maintained, allowing for a physiological stimulation via the apical route.

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Human Intestinal Mucosa Explants Intestinal Micro-environment Immunological Response Tolerance Against Food Antigens Immune Responses Against Pathogenic Microbes Microbiota-host Interactions Mucus Degradation Antimicrobial Peptides Epithelial Barrier Gut-resident Lymphoid Cells In Vitro Assays Mouse Models Irritable Bowel Syndrome Inflammatory Bowel Disease Colorectal Cancer
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