Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

Rafi Chapanian; Iren Constantinescu; Donald E. Brooks; Mark D. Scott; Jayachandran Kizhakkedathu

doi:10.3791/50075

Gli antigeni Protetto funzionali globuli rossi dalla membrana innesto di Compact Poligliceroli Hy...

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Immunology and Infection

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JoVE Journal Immunology and Infection

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

Gli antigeni Protetto funzionali globuli rossi dalla membrana innesto di Compact Poligliceroli Hyperbranched

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11:31 min

January 2, 2013

DOI: 10.3791/50075-v

Rafi Chapanian^1,2, Iren Constantinescu^1,3, Donald E. Brooks^1,2,4, Mark D. Scott^1,2,3, Jayachandran Kizhakkedathu^1,2,4

¹Centre for Blood Research,University of British Columbia , ²Department of Pathology and Laboratory Medicine,University of British Columbia , ³Canadian Blood Services,University of British Columbia , ⁴Department of Chemistry, Life Sciences Centre,University of British Columbia

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Overview

This study presents the modification of red blood cells (RBCs) using hyperbranched polyglycerol (HPG) to protect surface antigens. The modified RBCs were characterized through various assays, including flow cytometry.

Key Study Components

Area of Science

Cell membrane modification
Red blood cell functionality
Biomaterials in blood preservation

Background

Red blood cells play a crucial role in oxygen transport.
Surface antigens on RBCs are important for compatibility in transfusions.
Modification of RBC membranes can enhance their functionality.
HPG is a promising polymer for biomedical applications.

Purpose of Study

To prepare antigen-protected functional red blood cells.
To evaluate the effectiveness of HPG in protecting RBC surface proteins.
To assess the impact of HPG modification on RBC characteristics.

Methods Used

Preparation of HPG polymer with succinyl succinate groups.
Washing whole blood with saline to isolate RBCs.
Treatment of isolated RBCs with functionalized HPG.
Flow cytometry to assay surface antigen protection.

Main Results

Successful modification of RBCs with HPG was achieved.
Characterization revealed changes in osmotic fragility.
Complement-mediated lysis was assessed.
Flow cytometry confirmed the camouflage of surface proteins.

Conclusions

HPG effectively protects RBC surface antigens.
Modified RBCs may have improved functionality for transfusion.
This method could enhance the preservation of blood products.

Frequently Asked Questions

What is hyperbranched polyglycerol?

Hyperbranched polyglycerol is a type of polymer used for modifying cell membranes.

How are red blood cells modified in this study?

RBCs are modified by treating them with functionalized HPG to protect surface antigens.

What assays were used to characterize modified RBCs?

Assays included flow cytometry, osmotic fragility tests, and complement-mediated lysis assessments.

Why is protecting RBC surface antigens important?

Protecting surface antigens is crucial for ensuring compatibility in blood transfusions.

What are the potential applications of this research?

This research could lead to improved blood preservation techniques and enhanced transfusion outcomes.

La modifica membrana cellulare dei globuli rossi (RBC) con poliglicerolo hyperbranched (HPG) è presentato. RBC modificati sono stati caratterizzati da due partizionamento fase acquosa, la fragilità osmotica e lisi complemento mediata. Il camuffamento di proteine di superficie e degli antigeni è stata valutata utilizzando la citometria a flusso e sistema Micro Typing (MTS) carte fenotipizzazione sangue.

Lo scopo di questa procedura è quello di preparare globuli rossi funzionali protetti dall'antigene. Ciò si ottiene preparando prima il polimero HPG nella seconda fase. L'HPG viene funzionalizzato attraverso l'aggiunta di gruppi succinilsuccinato.

Successivamente, il sangue intero di donatori sani viene lavato con soluzione fisiologica per separare i globuli rossi. Nella fase finale, i globuli rossi isolati vengono trattati con l'HPG funzionalizzato. In definitiva, la protezione degli antigeni di superficie sulle cellule modificate da parte dell'HPG funzionalizzato viene analizzata mediante citometria a flusso.

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