Biochimica e High Throughput valutazione microscopica di massa grassa in<em> Caenorhabditis Elegans</em
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March 30, 2013
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Vi presentiamo robusti metodi biochimici e microscopiche per lo studio<em> Caenorhabditis elegans</em> Depositi lipidici. Un rapido, semplice, fissando-procedura di colorazione per l'imaging fluorescente goccia lipidica sfrutta le proprietà spettrali del colorante lipofilo rosso Nilo. Abbiamo poi presentare misurazione biochimica di trigliceridi e fosfolipidi mediante estrazione in fase solida e gas cromatografia-spettrometria di massa.
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Pino, E. C., Webster, C. M., Carr, C. E., Soukas, A. A. Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans. J. Vis. Exp. (73), e50180, doi:10.3791/50180 (2013).
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