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Immunology and Infection

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Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Article DOI: 10.3791/51030-v • 10:38 min • February 24th, 2014
February 24th, 2014 •
1,2, 1, 1, 1, 1, 1, 1,3, 4, 2, 1
1Department of Entomology, Volcani Center, 2Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, 3Department of Applied Sciences, Institute for Adriatic Crops and Karst Reclamation, 4The Institute of Plant Sciences, Volcani Center

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We describe here a simple fluorescence in situ hybridization (FISH) method for the localization of viruses and bacteria in insect and plant tissues. This protocol can be extended for the visualization of mRNA in whole mount and microscopic sections.

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Fluorescence In Situ Hybridization FISH Gene Transcripts Eukaryotic Cells Viruses Bacteria Infection Process Expression Profiling Experiments Microarrays RNAseq Spatiotemporal Infections Cellular Components Reporter Gene Systems Immunohistochemical Methods Model Organisms Diagnostic Purposes Short Hybridizing Probes
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