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Journal / Immunology and Infection / Fluorescenza
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Immunology and Infection

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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Journal (Immunology and Infection)
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
Journal (Immunology and Infection)
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
 
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Fluorescenza

Article DOI: 10.3791/51030-v • 10:38 min • February 24th, 2014
February 24th, 2014 •
1,2, 1, 1, 1, 1, 1, 1,3, 4, 2, 1
1Department of Entomology, Volcani Center, 2Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, 3Department of Applied Sciences, Institute for Adriatic Crops and Karst Reclamation, 4The Institute of Plant Sciences, Volcani Center

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Descriviamo qui un semplice ibridazione in situ fluorescente (FISH) metodo per la localizzazione di virus e batteri nei tessuti insetti e vegetali. Questo protocollo può essere esteso per la visualizzazione di mRNA in tutto il monte e le sezioni microscopiche.

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Infezione FISH localizzazione insetti piante virus endosymbiont trascrizione la fissazione la microscopia confocale
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