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Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
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Biologia
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JoVE Journal Biologia
Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

DOI:
10.3791/51190-v

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09:16 min

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January 30, 2014

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, ,
1Institute for Cell and Molecular Biology,The University of Texas at Austin

Capitoli

  • 00:05Titolo
  • 01:07Mounting Zebrafish Embryos for Confocal Microscopy
  • 04:15Define Experiment Settings
  • 07:09Results: Craniofacial Defects
  • 08:31Conclusion

Summary

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Time-lapse confocal imaging is a powerful technique useful for characterizing embryonic development. Here, we describe the methodology and characterize craniofacial morphogenesis in wild-type, as well as pdgfra, smad5, and smo mutant embryos.

Tags

Craniofacial MorphogenesisZebrafish4D Confocal MicroscopyTime-lapse ImagingFluorescent LabelingNeural CrestCraniofacial Development

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