Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / In vivo Interrogation of Central Nervous System…
JoVE Journal
Neuroscience

Questo Contenuto è ad Accesso gratuito.

JoVE Journal Neuroscience
In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish
Journal (Neuroscience)
Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish
Employing Transcranial Magnetic Stimulation in a Resource Limited Environment to Establish Brain-Behavior Relationships
Journal (Neuroscience)
Employing Transcranial Magnetic Stimulation in a Resource Limited Environment to Establish Brain-Behavior Relationships
 

In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation

Article DOI: 10.3791/51255-v • 09:13 min • April 30th, 2014
April 30th, 2014 •
1, 1, 1, 1
1Department of Molecular Neurobiology, German Cancer Research Center (DKFZ)

Capitoli

Riepilogo
Automatic Translation

This protocol illustrates essential modifications of polyribosome fractionation in order to study the translatome of in vivo CNS samples. It allows global assessment of translation and transcription regulation through the isolation and comparison of total RNA to ribosome bound RNA fractions.

Tags

In Vivo Central Nervous System Translatome Polyribosome Fractionation Gene Expression Transcription Translation MRNA Stability Protein Abundance Regulatory Mechanisms MRNA Translation Translatomics Neurobiology Polyribosome Fractionation Technique Actively Translated MRNAs Ribosomes Sucrose Gradients In Vivo Samples Central Nervous System (CNS) Fatty Tissue Components CNS Material Mouse Spinal Cord Brain Cycloheximide Myelin Flotation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter