L'isolamento a base di affinità di Tagged Nuclei di<em> Drosophila</em> tessuti per Gene Expression Analysis
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March 25, 2014
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Tessuti Drosophila spesso contengono una miscela eterogenea di tipi cellulari. Per esaminare l'espressione genica in specifici tipi cellulari da un particolare tessuto, i nuclei possono essere geneticamente marcato e successivamente isolate utilizzando un approccio basato affinità. Isolato nuclei possono essere utilizzate per applicazioni a valle come l'analisi dell'espressione genica e della cromatina.
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Cite this Article
Ma, J., Weake, V. M. Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis. J. Vis. Exp. (85), e51418, doi:10.3791/51418 (2014).
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