Rivista
/
Biologia
/
Le colture per pluripotenti umane alternative staminali produzione di celle, manutenzione e analisi genetica
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Le colture per pluripotenti umane alternative staminali produzione di celle, manutenzione e analisi genetica

Please note that all translations are automatically generated. Click here for the English version.

12,225 Views

•

08:27 min

•

July 24, 2014

DOI:

10.3791/51519-v

DOI:
10.3791/51519-v

•

08:27 min
July 24, 2014

•

8 Views
, , ,
1NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke,National Institutes of Health, 2Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research,National Institutes of Health

Summary

Automatically generated

Here, we present human pluripotent stem cell (hPSC) culture protocols, based on non-colony type monolayer (NCM) growth of dissociated single cells. This new method, utilizing Rho-associated kinase inhibitors or the laminin isoform 521 (LN-521), is suitable for producing large amounts of homogeneous hPSCs, genetic manipulation, and drug discovery.

Explore More Videos

Human Pluripotent Stem CellsHPSCsCell CultureNon-colony Type MonolayerNCMROCK InhibitorExtracellular MatrixGenetic ModificationTransfectionTransductionRegenerative MedicineBiopharmaceutical Applications

Read Article

Cite this Article

Chen, K. G., Hamilton, R. S., Robey, P. G., Mallon, B. S. Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis. J. Vis. Exp. (89), e51519, doi:10.3791/51519 (2014).

Download .ris file

Copy

Share Video

.

Copy