RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Houtan Moshiri; Vaibhav Mehta; Reza Salavati

doi:10.3791/51712

Rivista

/

Biologia

/

RNA Catalyst come reporter per lo screening dei farmaci contro l'RNA Editing in tripanosomi

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

JoVE Journal
Biologia

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biologia

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

RNA Catalyst come reporter per lo screening dei farmaci contro l'RNA Editing in tripanosomi

Please note that all translations are automatically generated. Click here for the English version.

8,397 Views

•

09:19 min

•

July 22, 2014

DOI:

10.3791/51712-v

DOI:

10.3791/51712-v

•

09:19 min

July 22, 2014

•

1 Views

Houtan Moshiri*^1,2, Vaibhav Mehta*^1,2, Reza Salavati^1,2,3

¹Department of Biochemistry,McGill University, ²Institute of Parasitology,McGill University, ³McGill Centre for Bioinformatics,McGill University

Summary

A highly sensitive ribozyme-based assay, applicable to high-throughput screening of chemicals targeting the unique process of RNA editing in trypanosomatid pathogens, is described in this paper. Inhibitors can be used as tools for hypothesis-driven analysis of the RNA editing process and ultimately as therapeutics.