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RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

Article DOI: 10.3791/51951-v • 11:13 min • November 1st, 2014
November 1st, 2014 •
1, 1
1Department of Cell and Developmental Biology, Universidade de São Paulo

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This video shows the basic steps for performing whole transcriptome analysis on dissected chick embryonic spinal cord samples after transfection with in ovo electroporation.

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RNA-Seq Analysis Differential Gene Expression Electroporation Chick Embryonic Spinal Cord In Ovo Electroporation Gene Function Neural Development Genome-wide Analysis Transfected Construct Transcriptomes RNA Samples Transcriptome Sequencing Bioinformatic Analysis Galaxy Public Server Computational Structure Dissection Methods High-quality RNA Samples
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