From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Carmine Di Rienzo; Enrico Gratton; Fabio Beltram; Francesco Cardarelli

doi:10.3791/51994

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Da Veloce imaging di fluorescenza di Molecular legge Diffusione su membrane cellulare dal vivo in un microscopio commerciale

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

JoVE Journal
Bioingegneria

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JoVE Journal Bioingegneria

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Da Veloce imaging di fluorescenza di Molecular legge Diffusione su membrane cellulare dal vivo in un microscopio commerciale

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11,413 Views

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15:10 min

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October 09, 2014

DOI:

10.3791/51994-v

DOI:

10.3791/51994-v

•

15:10 min

October 09, 2014

•

11389 Views

Carmine Di Rienzo^1,2, Enrico Gratton³, Fabio Beltram¹, Francesco Cardarelli²

¹NEST Laboratory,Scuola Normale Superiore ²Center for Nanotechnology Innovation,Instituto Italiano di Tecnologia ³Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering,University of California, Irvine

Summary

Spatial distribution and temporal dynamics of plasma membrane proteins and lipids is a hot topic in biology. Here this issue is addressed by a spatio-temporal image fluctuation analysis that provides conceptually the same physical quantities of single particle tracking, but it uses small molecular labels and standard microscopy setups.