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A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Article DOI: 10.3791/52161-v • 07:24 min • December 10th, 2014
December 10th, 2014 •
1, 2, 3, 4, 1, 5
1Egg Safety and Quality Research Unit, USDA-Agricultural Research Service, 2Poultry Microbiological Safety and Processing Research Unit, USDA-Agricultural Research Service, 3Department of Biochemistry and Biophysics, Oregon State University, 4College of Public Health, University of Georgia, 5Department of Biological Sciences, Center for Microbial Genetics and Genomics, Northern Arizona University

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A novel semi-automated hybrid DNA extraction method for use with environmental poultry production samples was developed and demonstrated improvements over a common mechanical and enzymatic extraction method in terms of the quantitative and qualitative estimates of the total bacterial communities.

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Hybrid DNA Extraction Method Qualitative Assessment Quantitative Assessment Bacterial Communities Poultry Production Samples DNA Extraction Protocols Downstream Analyses Microbiomics Metagenomics Environmental Sciences Physiochemical Uniqueness Microbiological Uniqueness Fecal Samples Litter/bedding Samples Poultry Production Spectrum Semi-automated Method Mechanical Homogenization Step Enzymatic Extraction Method Bead-beating Gold Standard Method Recovery Of Gram-positive Bacterial Community Members Purification Process Robotic Workstation
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