Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Vishal Menon; Ria Thomas; Arun R. Ghale; Christina Reinhard; Jan Pruszak

doi:10.3791/52241

Rivista

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Neuroscienze

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Citometria a flusso Protocolli per superficie e intracellulare Antigen analisi di tipi di cellule neurali

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Citometria a flusso Protocolli per superficie e intracellulare Antigen analisi di tipi di cellule neurali

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52,312 Views

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13:05 min

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December 18, 2014

DOI:

10.3791/52241-v

DOI:

10.3791/52241-v

•

13:05 min

December 18, 2014

•

52211 Views

Vishal Menon¹, Ria Thomas^1,2, Arun R. Ghale^1,3, Christina Reinhard¹, Jan Pruszak^1,4

¹Emmy Noether-Group for Stem Cell Biology, Department of Molecular Embryology, Institute of Anatomy and Cell Biology,University of Freiburg ²Spemann Graduate School of Biology and Medicine and Faculty of Biology,University of Freiburg ³School of Life Sciences,Keele University ⁴Center for Biological Signaling Studies (BIOSS),University of Freiburg

Summary

We provide a detailed description of a protocol for flow cytometric analysis of surface antigens and/or intracellular antigens in neural cell types. Critical aspects of experimental planning, step-by-step methodological procedures, and fundamental principles of flow cytometry are explained in order to enable neurobiologists to exploit this powerful technology.