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Immunology and Infection

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Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes

Article DOI: 10.3791/52416-v • 10:01 min • February 11th, 2015
February 11th, 2015 •
*1, *1, 2, 1, 1, 1,2, 1,4,5, 3,5, 1,4,5
1Department of Neurosurgery, UCLA David Geffen School of Medicine, 2Department of Molecular and Medical Pharmacology, UCLA David Geffen School of Medicine, 3Department of Medicine, UCLA David Geffen School of Medicine, 4Brain Research Institute, UCLA David Geffen School of Medicine, 5Jonsson Comprehensive Cancer Center, UCLA David Geffen School of Medicine
* These authors contributed equally

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We describe a protocol to monitor radial mobility of non-adherent immune cells in vitro using a cell sedimentation manifold/slide apparatus. Cell migration is tracked on monolayers of tumor cells or on extracellular matrix proteins. Examination by light and fluorescence microscopy allows for observation of cell mobility and cytotoxic functionality.

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Radial Mobility Cytotoxic Function Retroviral Replicating Vector Transduced T Lymphocytes Non-adherent T Cells Radial Monolayer Cell Migration Assay Motility Fluorescent Labeling Effector Immune Cells Confluent Tumor Cell Monolayers Extracellular Matrix Proteins Fluorescence Microscopy Fluorescent Dyes Viral Vectors Imaging Data Horizontal Migration/invasion Slide Chambers Agar Plates Transwell Plates Specific Fluorescent Markers Surface Intensity Fluorescence Plots
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