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Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer

Article DOI: 10.3791/52542-v • 08:25 min • March 23rd, 2015
March 23rd, 2015 •
*1, *1, 1, 2, 3, 1
1Department of Cell and Molecular Physiology, Loyola University Chicago, 2Department of Biomedical Engineering, University of Alabama at Birmingham, 3Department of Bioengineering, Clemson University
* These authors contributed equally

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This article describes the technique used to perform dual channel optical mapping in cultured HL-1 atrial cell monolayers. This unique protocol allows the simultaneous visualization of both calcium (Ca) and voltage (Vm) activity in the same area for the detailed detection and analysis of electrophysiological properties of culture monolayers.

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Voltage Calcium Dual Channel Optical Mapping Cultured HL-1 Atrial Myocyte Monolayer Cardiac Electrical Activity Ex Vivo Hearts Cultured Myocyte Monolayers HL-1 Cells 2-Dimensional Cellular Model Cardiac Physiology Calcium Transients Action Potentials Optically Mapped Optimal Working Protocol Action Potential Propagation Ca Kinetics Arrhythmia Development
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