Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons

Chongbo Zhong; David A. Talmage; Lorna W. Role

doi:10.3791/52730

Rivista

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Neuroscienze

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Immagini dal vivo di nicotina indotta segnalazione Calcio e neurotrasmettitori uscita Lungo ventrali Hippocampal assoni

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons

Immagini dal vivo di nicotina indotta segnalazione Calcio e neurotrasmettitori uscita Lungo ventrali Hippocampal assoni

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8,935 Views

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12:19 min

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June 24, 2015

DOI:

10.3791/52730-v

DOI:

10.3791/52730-v

•

12:19 min

June 24, 2015

•

1 Views

Chongbo Zhong¹, David A. Talmage², Lorna W. Role¹

¹Department of Neurobiology and Behavior,Stony Brook University, ²Department of Pharmacological Science,Stony Brook University

Summary

We developed a gene-chimeric preparation of ventral hippocampal – accumbens circuit in vitro that allows direct live imaging to analyze presynaptic mechanisms of nicotinic acetylcholine receptors (nAChRs) mediated synaptic transmission. This preparation also provides an informative approach to study the pre- and post-synaptic mechanisms of synaptic plasticity.