Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Laura M. Ketelboeter; Sonia L. Bardy

doi:10.3791/53105

/

Metodi Per inibire batterica Pyomelanin produzione e determinare il corrispondente aumento di sensibilità allo stress ossidativo

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

JoVE Journal
Immunologia e infezioni

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunologia e infezioni

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Metodi Per inibire batterica Pyomelanin produzione e determinare il corrispondente aumento di sensibilità allo stress ossidativo

Please note that all translations are automatically generated. Click here for the English version.

8,129 Views

•

11:00 min

•

August 31, 2015

DOI:

10.3791/53105-v

DOI:

10.3791/53105-v

•

11:00 min

August 31, 2015

•

1 Views

Laura M. Ketelboeter¹, Sonia L. Bardy¹

¹Department of Biological Sciences,University of Wisconsin Milwaukee

Summary

Bacterial pyomelanin production results in increased resistance to oxidative stress and virulence. We report on techniques that can be used to determine inhibition of pyomelanin production and assay the resulting increase in sensitivity to oxidative stress in bacteria, as well as determine antibiotic minimum inhibitory concentration (MIC).