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Immunology and Infection

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Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry

Article DOI: 10.3791/53372-v • 07:25 min • November 4th, 2015
November 4th, 2015 •
1, 1
1Institute of Medical Virology, University of Zurich

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We present a protocol describing a semi-quantitative method for measuring both, the attachment of influenza A virus to A549 cells, as well as the internalization of virus particles into the target cells by flow cytometry.

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Attachment Internalization Influenza A Virus A549 Cells Flow Cytometry Biotinylated Virus Cy3-labeled Streptavidin Viral Particles Growth Protocol Purification Biotinylation Laboratory Strain Cold-bound Virus Flow Cytometry Measurement Uptake Blocking Step Cell Permeabilization Drug Treatments Manipulations
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