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Genoombrede kartering van Drug-DNA interacties in cellen met kosmische (Crosslinking van kleine moleculen te isoleren chromatine)

Article DOI: 10.3791/53510-v • 10:05 min • January 20th, 2016
January 20th, 2016 •
1, 1, 1,2, 3, 1, 1,4
1Department of Biochemistry, University of Wisconsin–Madison, 2Department of Electrical and Computer Engineering, University of Wisconsin–Madison, 3Graduate Program in Cellular and Molecular Biology, University of Wisconsin–Madison, 4The Genome Center, University of Wisconsin–Madison

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Het identificeren van de directe doelstellingen van genoom-gerichte moleculen blijft een grote uitdaging. Om te begrijpen hoe DNA-bindende moleculen die aangrijpen op de genoom, ontwikkelden we een methode die is gebaseerd op de verknoping van kleine moleculen aan chromatine isoleren (COSMIC).

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Molecular Biology kosmisch CSI moleculaire herkenning cheminformatica chemische genomics polyamide genoom targeting precisie geneeskunde DNA chromatine immunoprecipitatie next-generation sequencing
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