Radiomarcatura e quantificazione dei livelli cellulari di fosfoinositidi da alte prestazioni cromatografia liquida accoppiata flusso scintillazione
10,449 Views
•
10:52 min
•
January 06, 2016
DOI:
Summary
Automatically generated
Fosfoinositidi stanno segnalando lipidi la cui abbondanza relativa cambia rapidamente in risposta a vari stimoli. Questo articolo descrive un metodo per misurare l'abbondanza di fosfoinositidi dalle cellule metabolicamente marcatura con 3 H- myo-inositolo, seguita da estrazione e deacilazione. Estratti glycero-inositides sono quindi separati mediante cromatografia liquida ad alta prestazione e quantificati dal flusso scintillazione.
Video correlati
07:26
Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
Video correlati
9551 Views
08:38
Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
Video correlati
21280 Views
08:07
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
Video correlati
8554 Views
09:01
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
Video correlati
7056 Views
08:49
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
Video correlati
4053 Views
09:22
Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
Video correlati
2288 Views
08:26
Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
Video correlati
2439 Views
10:58
PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
Video correlati
9502 Views
11:32
Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
Video correlati
13296 Views
08:28
Metabolic Labeling
Video correlati
12384 Views
Read Article
Cite this Article
Ho, C. Y., Choy, C. H., Botelho, R. J. Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation. J. Vis. Exp. (107), e53529, doi:10.3791/53529 (2016).
Copy