Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy

Gabriele Di Sante; Mathew C. Casimiro; Timothy G. Pestell; Richard G. Pestell

doi:10.3791/53657

Rivista

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Biologia

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Time-Lapse Video Microscopia per la valutazione di EYFP-Parkin aggregazione come marcatore per cellulare Mitophagy

Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy

JoVE Journal
Biologia

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JoVE Journal Biologia

Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy

Time-Lapse Video Microscopia per la valutazione di EYFP-Parkin aggregazione come marcatore per cellulare Mitophagy

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7,126 Views

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09:29 min

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May 04, 2016

DOI:

10.3791/53657-v

DOI:

10.3791/53657-v

•

09:29 min

May 04, 2016

•

1 Views

Gabriele Di Sante¹, Mathew C. Casimiro¹, Timothy G. Pestell², Richard G. Pestell^1,2,3

¹Department of Cancer Biology,Thomas Jefferson University, SKCC, ²Department of Medical Oncology,Thomas Jefferson University, SKCC, ³Kazan Federal University

Trascrizione

Summary

Herein, we describe in detail a time-lapse video microscopy approach to measuring the temporal recruitment of EYFP-Parkin during the selective removal of damaged mitochondria. This dynamic process of EYFP-Parkin-dependent removal of damaged mitochondria can be used as an indicator of cellular health under different experimental conditions.