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Developmental Biology

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JoVE Journal Developmental Biology
Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
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Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells

Article DOI: 10.3791/53692-v • 12:17 min • January 2nd, 2016
January 2nd, 2016 •
1, *1, *1, 1, 1
1Cell Biology & Neuroscience, Rutgers University
* These authors contributed equally

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The current protocols to maintain immortalized multipotent otic progenitor (iMOP) cells and otic differentiation are described. Culture conditions and molecular markers that indicate differentiation into sensory epithelia and spiral ganglion neurons (SGN) are highlighted.

Tags

Differentiation Immortalized Multipotent Otic Progenitor Cells Inner Ear Cell Types Auditory Regeneration Cochlear Hair Cells Auditory Neurons In-vitro Cellular System In-vivo Mammalian Animal Model Systems Culture Medium IMOP Cells Tissue Culture Dish Incubate Carbon Dioxide Passage Cells Conical Tube Otospheres
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