Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Florence A. Giger; Julien G. Dumortier; Nicolas B. David

doi:10.3791/53792

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analizzare In Vivo Migrazione cellulare utilizzando Trapianti cellulari e Time-lapse imaging in embrioni di zebrafish

Analyzing <em>In Vivo</em> Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

analizzare In Vivo Migrazione cellulare utilizzando Trapianti cellulari e Time-lapse imaging in embrioni di zebrafish

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7,611 Views

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11:39 min

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April 29, 2016

DOI:

10.3791/53792-v

DOI:

10.3791/53792-v

•

11:39 min

April 29, 2016

•

1 Views

Florence A. Giger¹, Julien G. Dumortier², Nicolas B. David¹

¹CNRS UMR8197 – INSERM U1024,IBENS, Institut de Biologie de l’École Normale Supérieure, ²Department of Physiology Development and Neuroscience,University of Cambridge

Trascrizione

Summary

Combining cell transplantation, cytoskeletal labeling and loss/gain of function approaches, this protocol describes how the migrating zebrafish prospective prechordal plate can be used to analyze the function of a candidate gene in in vivo cell migration.