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Journal / Developmental Biology / Cultivate Primary Nasal Epithelial Cells from…
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Developmental Biology

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Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
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Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

Article DOI: 10.3791/53814-v • 12:08 min • March 10th, 2016
March 10th, 2016 •
1, 2, 3, 4, 1,4
1Pyrosequencing Core, Cincinnati Children's Hospital, 2Division of Developmental Biology, Cincinnati Children's Hospital, 3Division of Pulmonary Medicine, Seattle Children's Hospital, 4Division of Asthma Research, Cincinnati Children's Hospital

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This publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).

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Nasal Epithelial Cells Children Induced Pluripotent Stem Cells Aerated Disease Asthma Airway Epithelial Cells Homogeneous Population IPSCs Epigenetic Variation Asthma And Air Pollution Nasal Sampling Reprogramming Bronchial Epithelial Growth Medium BEGM Penicillin Streptomycin Amphotericin B Cytology Brush
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