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Genetics

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siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Article DOI: 10.3791/53995-v • 09:57 min • September 20th, 2016
September 20th, 2016 •
1, 2, 3, 1
1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal

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This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.

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SiRNA Transfection EMSA Analysis Freshly Isolated Human Villous Cytotrophoblasts DNA Protein Interaction Promoter Sites Placenta Research Cytotrophoblast Differentiation Regulatory Mechanisms Easy To Perform Efficient FACS Analysis Purity Analysis Micro Centrifuge Tubes PBS Methanol Incubation
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