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Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
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Biologia
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JoVE Journal Biologia
Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products

Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products

DOI:
10.3791/54281-v

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05:27 min

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December 25, 2016

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, ,
1Department of Pediatrics & Human Development, Institute for Integrative Toxicology,Michigan State University, 2RECETOX — Research Centre for Toxic Compounds in the Environment, Faculty of Science,Masaryk University

Capitoli

  • 00:05Titolo
  • 00:50SL-DT Bioassay
  • 02:49Loading the Dye
  • 04:17Results: Visualization and Analysis of Toxicant-induced GJIC Inhibition
  • 04:59Conclusion

Summary

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This protocol describes a scalpel loading-fluorescent dye transfer technique that measures intercellular communication through gap junction channels. Gap junctional intercellular communication is a major cellular process by which tissue homeostasis is maintained and disruption of this cell signaling has adverse health effects.

Tags

Gap Junctional Intercellular CommunicationToxicologyToxicantsToxinsNatural ProductsIn VitroPharmacologyBiomarkerWB-F344 Rat Liver Epithelial CellsDose-responseTime-responsePhenanthreneAcetonitrileCell CultureCell ConfluenceCell Treatment

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