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Olfactory Behaviors Assayed by Computer Tracking Of Drosophila in a Four-quadrant Olfactometer

Article DOI: 10.3791/54346-v • 08:52 min • August 20th, 2016
August 20th, 2016 •
1, 2, 1
1The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University School of Medicine, 2MRC Clinical Sciences Center, Imperial College London

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We describe here a behavioral setup and data analysis method for assaying olfactory responses of up to 100 vinegar flies (Drosophila melanogaster). This system may be used with single or multiple olfactory stimuli, and adaptable for optogenetic activation or silencing of neuronal subsets.

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Olfactory Behaviors Computer Tracking Drosophila Four-quadrant Olfactometer Automated Fly Tracking Software Script Olfactory Neural Systems Behavioral Decisions Versatile And Robust Technique Repeat Testing Large Experimental Arena Odorant Chambers Plastic Outer Container Glass Inner Container Custom-made PTFE Lid Insert Solvent Controls Test Odorant Clean Chamber Odorant Container Solvent Or Odorant Dilution Position Of The Container Prevent Leaks Rearing Flies Cornmeal Medium
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