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Methods for the Determination of Rates of Glucose and Fatty Acid Oxidation in the Isolated Working Rat Heart
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Methods for the Determination of Rates of Glucose and Fatty Acid Oxidation in the Isolated Working Rat Heart

Article DOI: 10.3791/54497-v • 12:24 min • September 28th, 2016
September 28th, 2016 •
1, 1, 1
1Department of Physiology and Biophysics, Mississippi Center for Obesity Research, Cardiovascular-Renal Research Center, University of Mississippi Medical Center

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The following protocol describes the preparation and utilization of buffers for the quantitative measurement of rates of glucose and fatty acid oxidation in the isolated working rat heart. The methods used for sample analysis and data interpretation are also discussed.

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Glucose Oxidation Fatty Acid Oxidation Isolated Working Rat Heart Cardiovascular Field Myocardial Rates Energy Demands Pathological Conditions Environmental Variables Substrates Drugs Hormones Perfusion Apparatus Surgery Heparin Diaphragm Rib Cage Chest Cavity
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